bioinfo.appliedbiosystems.comTaqMan Search

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Our collection covers long ncRNA transcripts from sources including NCBI RefSeq NR, RNAdb, and content on Invitrogen™ NCode™ Non-coding RNA Arrays. Markers & Reporters Markers & Reporters Marker & Reporter assays can be used to verify and quantify reporter gene products from expression vectors that have been introduced into cells or animals, such as lacZ or GFP. Controls Controls Control Assays target commonly used endogenous control or reference RNAs. These Control Assays are typically used for normalization for relative gene expression quantitation data. Fusion Fusion TaqMan™ Gene Expression Fusion Assays are designed to detect fusion transcripts using real-time PCR. Our collection covers gene fusion transcripts from sources including NCBI Genbank and content on Oncomine™ products. Microbe Detection What type of assay do you want? All SNP Genotyping SNP Genotyping Assays Search all TaqMan™ SNP Genotyping Assays, including Drug Metabolism Genotyping Assays, and assays that are functionally tested or validated. Drug Metabolism Assays Drug Metabolism Assays These assays are specifically designed to detect polymorphisms in 221 genes that code for various drug metabolism enzymes and drug transporters. Assays are tested using gDNA from 45 individuals from four ethnicities. The minor allele frequency for each population is available, but rare and mutant alleles may not be present in the test DNA. Which Copy Number product(s) are you interested in using? All Copy Number Assays All Copy Number Assays Search all TaqMan™ Copy Number Assays and required TaqMan™ Copy Number Reference Assays. Markers & Reporters Markers & Reporters Marker & Reporter assays are designed to detect the copy number of marker and reporter genes from expression vectors that have been introduced into cells or animals, such as the lacZ reporter gene or antibiotic genes. What type of siRNA do you want? Silencer ® Select siRNA Recommended Use: Cell culture RNAi experiments Silencer ® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNA with unrivaled efficacy, potency, and specificity. They are recommended for in vitro RNAi experiments. Ambion ® In Vivo siRNA Recommended Use: Animal experiments Ambion ® In Vivo siRNAs are designed using the Silencer ® Select algorithm and incorporate chemical modifications that help provide superior serum stability for in vivo delivery. Stealth RNAi™ siRNA Recommended Use: Continuity in cell culture and animal experiments Stealth RNAi siRNAs uses their proprietary chemical modifications for restricting off-target effects and their effective algorithm for providing efficient knockdown. They are recommended for completing in vitro RNAi studies that were started using Stealth siRNAs. For new studies, we recommend Ambion Silencer Select siRNAs. Unmodified siRNA Recommended Use: Continuity in cell culture experiments Silencer ® siRNAs are designed using our first-generation algorithm and do not include any chemical modifications. They are recommended for completing in vitro RNAi studies that were started using Silencer ® siRNA. For new studies, we recommend Silencer ® Select siRNAs. Which miRNA product(s) are you interested in using? TaqMan™ Advanced miRNA Assays NEW TaqMan™ Advanced miRNA Assays TaqMan™ Advanced miRNA Assays, based on a universal RT approach, provide even greater sensitivity in biological fluids and proven TaqMan™ specificity. TaqMan™ MicroRNA Assays TaqMan™ miRNA Assays TaqMan™ MicroRNA Assays, based on the specific stem-loop primer approach, are the gold standard for sensitivity and specificity. Controls Controls The miRNA control assays are unique to each assay chemistry type (universal RT or miRNA- specific stem loop RT) and cannot be used with the other assay chemistry. TaqMan™ Pri-MicroRNA Assays Primary MicroRNA Assays TaqMan™ Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the initial RNA molecules in the microRNA (miRNA) biogenesis pathway. Mimics/Inhibitors Mimics Small, synthetic, double-stranded RNA molecules designed to mimic endogenous mature miRNAs. They are supplied ready-to-use; they can be introduced into cells using transfection or electroporation. We recommend mir Vana ™ miRNA Mimics, but we continue to offer Ambion ® Pre-miR miRNA Precursors for completion of studies that were started using these first-generation products. Inhibitors Small, synthetic, single-stranded RNA molecules designed to specifically bind to and inhibit mature miRNAs from functioning. We recommend mir Vana™ miRNA inhibitors, but we continue to offer Ambion ® Anti-miR™ miRNA Inhibitors for completion of studies that were started using these first-generation products. Which technology are you using? Digital PCR Mutation Detection: Digital PCR Search all Mutation Detection Assays using Digital PCR TaqMan™ Technology. Real-Time PCR Mutation Detection: Real-Time PCR Search all Mutation Detection Assays using Real-Time PCR TaqMan™ Technology. What Array format do you want? All Arrays All Arrays Search all array formats, from 96-well to OpenArray® format (3072 throughholes) 96-well standard 96-well standard Sets of pre-selected TaqMan™ Gene Expression assays for specific pathways, biomarker sets, or disease states in 96-well plate format 96-well Fast 96-well Fast The same sets of pre-selected TaqMan™ Gene Expression assays as our 96-well standard arrays, but optimized for Fast PCR conditions. A Fast PCR-compatible thermal block is required. 384-well 384-well Sets of pre-selected TaqMan™ Gene Expression assays for specific pathways, biomarker sets, or disease states in 384-well microfluidic card format. A TaqMan™ Array block, available for our 7900HT, Viia7™ and QuantStudio™ 12K Flex systems, is required for OpenArray OpenArray Sets of pre-selected TaqMan™ Gene Expression assays for specific pathways, biomarker sets, or disease states in OpenArray™ format. A QuantStudio™ 12K Flex or NT Cycler system is required for these arrays. What Array format do you want? All Arrays All Arrays Search all array formats, from 96-well to OpenArray® format (3072 throughholes) 96-well standard 96-well standard Sets of pre-selected TaqMan™ MicroRNA assays for specific pathways, biomarker sets, or disease states in 96-well plate format 96-well Fast 96-well Fast The same sets of pre-selected TaqMan™ MicroRNA assays as our 96-well standard arrays, but optimized for Fast PCR conditions. A Fast PCR-compatible thermal block is required. 384-well 384-well Sets of pre-selected TaqMan™ MicroRNA assays for specific pathways, biomarker sets, or disease states in 384-well microfluidic card format. A TaqMan™ Array block, available for our 7900HT, Viia7™ and QuantStudio™ 12K Flex systems, is required for OpenArray OpenArray Sets of pre-selected TaqMan™ MicroRNA assays for specific pathways, biomarker sets, or disease states in OpenArray™ format. A QuantStudio™ 12K Flex or NT Cycler system is required for these arrays. ...